Our analyses are based on two technological standards of allergy molecular diagnosis:
ELISA (Enzyme-Linked Immunosorbent Assays) using a monoclonal antibody specific for measuring IgE present in serum.
Immunoblot tests identify in the allergenic source each protein capable of reacting with the IgE present in dog or cat serum.
In practice, sample proteins are extracted, separated on a gel according to their molecular weight and then transferred to a membrane (blot), which is then incubated in the presence of serum.
When antibodies against these proteins are present, they bind to the corresponding protein and a specific band appears on the membrane.